CONTAMINATION DURING MOLECULAR-GENETIC RESEARCH. ITS CAUSES AND CONSEQUENCES

Abstract

The author of the article highlights the problem of contamination that occurs during molecular-genetic researches (contamination of objects of biological origin with foreign DNA, which may lead to false results and affect the consequences of pre-trial investigation). The concept of contamination is explained. The sources of its origin are structured and the types are pointed out. The author emphasizes on potentially dangerous sources of contamination. The author emphasizes on potentially dangerous sources of contamination and describes the ways to detect and prevent them. The means of contamination control, which will prevent the receipt of pseudo-positive and pseudo-negative results during the establishment of human genetic features, are proposed. The algorithms of the methods application of preventing the
occurrence of contamination, its detection and elimination have been developed. These algorithms are considered to be effective tools for performance of the qualitative expertise of the researched objects held by the experts biologists. Recommendations on control measures in case of contamination occurrence before, during and after the molecular-genetic research are also developed in the article.
Key words: contamination; laboratory; molecular-genetic research; deoxyribonucleic acid.