PIPECURONIUM IDENTIFICATION ACCORDING TO THE SCHEME OF APPLICATION OF ANALYTICAL METHODS RESEARCH DEPENDING ON THEIR SELECTIVITY (SWGDRUG)

Abstract

The purpose of the article is to comprehensively analyze the theoretical and practical aspects of pipecuronium identification in accordance with the scheme of application of analytical research methods due to their selectivity, and to develop a method of testing a standard sample of pipecuronium by physicochemical methods to confirm substantiated expert report. Methodology. The reliability of the obtained results and conclusions is ensured by the use of a set of general scientific and special research methods. Methods of analysis, synthesis, comparison, generalization allowed
to analyze information sources in the field of research, as well as the analytical scheme of a set of physicochemical research methods recommended by SWGDRUG. Approbational analytical study of a standard sample of pipecuronium using experimental methods, analysis, comparison, as well as special physical, chemical, statistical research methods allowed to test a set of physicochemical methods of research of this substance and draw conclusions about
the suitability of certain species and outline further research. Scientific novelty. The laboratory of the State Research Forensic Center of the Ministry of Internal Affairs of Ukraine tested the analytical scheme of physicochemical research methods for identification of potent and toxic drugs, recommended by SWGDRUG, and proposed a method of testing a standard sample of pipecuronium by physicochemical methods according to the scheme of analytical methods. due to their selectivity. Conclusions. The SWGDRUG recommendations on the combination of analytical methods are analyzed and the minimum requirements for their application are characterized. It was stressed that in the context
of sufficiency for the identification of potent and toxic drugs, in particular heavy substances, expert laboratories, given the physicochemical properties of such substances and analytical equipment available in expert institutions
of the country, must determine a combination of methods to ensure reliable results. Analytical research.
The possibility of its identification by means of methods: qualitative chemical reactions (with the most available reagents, such as: Marquis, cobalt rhodonite, Dragendorff, Wagner, potassium iodoplatinate, Nessler), IR spectroscopy, mass spectrometry, Liquid chromatography–mass spectrometry with corona discharge detection. The expediency of combining methods is argued, which allows to implement the application of the analytical scheme of research methods recommended by SWGDRUG. It is stated that, given that the use of category B methods is not publicly available, the development of a derivatization process for the study of pipecuronium derivatives by publicly available methods: Gas chromatography-mass spectrometry (GC-MS) and Thin-layer chromatography using different types of sorbents. The results obtained during the approbation of a complex analytical study of pipecuronium according
to the recommended international scheme are summarized, and certain proposals are initiated, which can be
the basis of research methods of standard sample of pipecuronium by physicochemical methods to confirm
the validity, reliability, reproducibility of scientific identification expert report.