SIMULTANEOUS DETERMINATION OF THE CONTENT OF SYNTHETIC DYES Е110 AND Е124 IN THE MIXTURE

Abstract

The purpose of the article is to find out the possibility of using the method of zero cross section spectrophotometry of first derivatives to simultaneously determine the content of synthetic dyes E110 and E124 in the mixture without prior separation and develop a highly sensitive method of their control in soft drinks. Methodology. A set of general scientific and special methods was used to achieve this goal. In particular, theoretical materials on the research problem were systematized, while using theoretical methods (analysis and generalization, synthesis, comparison, modeling). The authors also used empirical method (diagnostic, methods of observation, self-observation) to determine the state of practical elaboration of the problem; and organizational-experimental methods (diagnostic, ascertaining, search, forming, corrective experiments) with qualitative analysis and mathematical statistical processing to prove the effectiveness of the proposed method. The reliability of the obtained results is ensured by the use of modern physicochemical, chemical, mathematical, statistical methods of analysis, as well as adequate processing of the obtained experimental data. Their accuracy is proved by comparison with the results of independent methods. Scientific novelty. The method of zero cross section spectrophotometry of first derivatives was introduced for unambiguous determination of E110 and E124 in model mixtures and in real mixtures and used to determine the content of dyes in soft drinks. Quantitative content of E110 and E124 dyes in non-alcoholic carbonated drinks «Mirinda», «Orange» and «Juicy Cherry» was determined. It was proved that the correctness of the determination depends on the concentration of each component and their ratios. It was also proved that the error of determination does not exceed 7 %, and the matrix of the object of analysis does not significantly affect the obtained results. Conclusions. Taking into account the availability of equipment, simplicity, expressiveness, seriation and workability of the proposed method for determining the content of individual dyes in the mixture without prior separation, the use of derivative spectrophotometry zero cross section of the first derivatives is an effective method of expert control of the content of synthetic dyes in soft drinks, syrups, etc. objects of forensic examination (soft drinks). The developed technique was tested on the model